HUMORAL IMMUNOLOGICAL PARAMETERS IN SMOKING AND NON-SMOKING PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE

Quantitative changes in immunoglobulins A (IgA), IgE, IgG, IgM, and B lymphocytes in peripheral blood were estimated in non-smoking and smoking patients with chronic obstructive pulmonary disease (COPD). The study included 21 non-smoking patients with COPD, 20 smoking patients with COPD, 20 healthy non-smokers, and 21 healthy smokers. The plasma immunoglobulin concentrations were measured by enzyme immunoassay. A B-lymphocyte population was analyzed by flow cytometry. In the smokers with COPD, IgA and IgE levels were significantly higher than those in the smokers without COPD, as well as in the non-smoking patients with COPD versus the healthy non-smokers. An increase in plasma IgG levels occurred only in the smoking patients with COPD. There were no differences in IgM and B lymphocyte levels in both smoking and non-smoking patients with COPD versus the respective groups of healthy individuals. The smoking patients with COPD showed a moderate positive correlation between total plasma IgE levels and smoking index. The findings suggest that IgA, IgE, and IgG are of pathogenetic value.

IgA, IgE, chronic obstructive pulmonary disease, immunoglobulins, non-smokers, smoking index, IgA, IgE

1. Брыляева Е. В., Крюков Н. Н., Жестков А. В. Иммунологические аспекты при патологии легких // Молодой ученый. - 2011. - № 1. - С. 243-244.

2. Кадушкин А. Г., Таганович А. Д., Лаптева И. М. Эпидемиологические особенности хронической обструктивной болезни легких у городских жителей Республики Беларусь // Здравоохранение. - 2013. - № 7. - С. 21-25.

3. Кадушкин А. Г., Таганович А. Д. Молекулярно-клеточные механизмы развития хронической обструктивной болезни легких // Воен. медицина. - 2012. - № 1. - С. 132-138.

4. Перельмутер В. М., Одинцов Ю. Н. Основная функция иммуноглобулинов класса М (IgM) - блокада преодоления бактериями гематотканевого барьера // Бюллетень сиб. мед. - 2005. - № 3. - С. 38-43.

5. Хайдуков С. В., Зурочка А. В., Черешев В. А. Цитометрический анализ в клинической иммунологии. - Екатеринбург, 2011. - 220 с.

6. Aaku E., Sorsa T., Wilkström M. Human immunoglobulin G potentiates superoxide production induced by chemotactic peptides and causes degranulation in isolated human neutrophils // Biochim. Biophys. Acta. - 1990. - Vol. 1052, № 2. - P. 243-247.

7. Ablin R. J. The elevation of serum IgA in emphysema // Am. Rev. Respir. Dis. - 1972. - Vol. 106. - P. 283-284.

8. Anthonisen N. R., Manfreda J., Warren C. P. et al. Antibiotic therapy in exacerbations of chronic obstructive pulmonary disease // Ann. Intern. Med. - 1987. - Vol. 106, № 2. - P. 196-204.

9. Barnes P. J. Emerging pharmacotherapies for COPD // Chest. - 2008. - Vol. 134. - P. 1278-1286.

10. Barnes P. J. The cytokine network in chronic obstructive pulmonary disease // Am. J. Respir. Cell Mol. Biol. - 2009. - Vol. 41. - P. 631-638.

11. Brandsma C. A., Hylkema M. N., Geerlings M. et al. Increased levels of (class switched) memory B cells in peripheral blood of current smokers // Respir. Res. - 2009. - Vol. 10. - Р. 108.

12. Chauhan S., Gupta M. K., Goyal A. et al. Alterations in immunoglobulin & complement levels in chronic obstructive pulmonary disease // Indian J. Med. Res. - 1990. - Vol. 92. - P. 241-245.

13. de Jong J. W., van der Belt-Gritter B., Koeter G. H. et al. Peripheral blood lymphocyte cell subsets in subjects with chronic obstructive pulmonary disease: association with smoking, IgE and lung function // Respir. Med. - 1997. - Vol. 91, № 2. - P. 67-76.

14. Domagała-Kulawik J., Hoser G., Dabrowska M. et al. CD4+/CD25+ cells in systemic inflammation in COPD // Scand. J. Immunol. - 2011. - Vol. 73, № 1. - P. 59-65.

15. Global health estimates summary tables: projection of deaths by cause, age and sex. Accessed 01/12/13 at: http://www.who.int/healthinfo/global_burden_disease/en/

16. Horton R. E., Vidarsson G. Antibodies and their receptors: different potential roles in mucosal defense // Front. Immunol. - 2013. - Vol. 4: 200.

17. Karnak D., Beder S., Kayacan O. et al. IgG subclass levels in ELF in COPD // Turk. J. Med. Sci. - 2001. - Vol. 31. - P. 235-241.

18. Miller R. D., Gleich G. J., Offord K. P. et al. Immunoglobulin concentrations in serum and nasal secretions in chronic obstructive pulmonary disease. A matched-pair study // Am. Rev. Respir. Dis. - 1979. - Vol. 119, № 2. - P. 229-238.

19. O’Keeffe S., Gzel A., Drury R. et al. Immunoglobulin G subclasses and spirometry in patients with chronic obstructive pulmonary disease // Eur. Respir. J. - 1991. - Vol. 4. - P. 932-938.

20. Pesci A., Balbi B., Majori M. et al. Inflammatory cells and mediators in bronchial lavage of patients with chronic obstructive pulmonary disease // Eur. Respir. J. - 1998. - Vol. 12. - P. 380-386.

21. Pilette C., Durham S. R., Vaerman J. P. et al. Mucosal immunity in asthma and chronic obstructive pulmonary disease. A role for Immunoglobulin A? // Proc. Am. Thorac. Soc. - 2004. - Vol. 1. - P. 125-135.

22. Salvi S. S., Barnes P. J. Chronic obstructive pulmonary disease in non-smokers // Lancet. - 2009. - Vol. 374. - P. 733-743.

23. Shopland D. R., Hartman A. M., Gibson J. T. et al. Cigarette smoking among U.S. adults by state and region: estimates from the current population survey // J. Natl. Cancer. Inst. - 1996. - Vol. 88. - P. 1748-1758.

24. Stone K. D., Prussin C., Metcalfe D. D. IgE, mast cells, basophils, and eosinophils // J. Allergy Clin. Immunol. - 2010. - Vol. 125 (Suppl. 2). - P. S73-S80.

25. Wanger J., Clausen J. L., Coates A. et al. Standartisation of the measurement of lung volumes // Eur. Respir. J. - 2005. - Vol. 26, № 3. - P. 511-522.