DETECTION OF TISSUE MYCOBACTERIUM TUBERCULOSIS BY DIFFERENTIATING IMMUNOPEROXIDASE STAINING
https://doi.org/10.21292/2075-1230-2014-0-10-50-54
Abstract
Staining impression smears from organ and tissues with peroxidase conjugated antibodies to Mycobacterium tuberculosis complex antigens, followed by visualization with diaminobenzidine and Kinyoun stains, ensured the painting of acid-resistant Mycobacterium tuberculosis forms to rubin red, acid-susceptible ones to brown, and tissue cells and microorganisms of other species to blue. Typical bacilli were absent in the lymph nodes of patients and animals with latent infection, but acid-resistant (rubin-red) granular forms were encountered in the granulomatous masses. Brown fat cells containing mycobacterial antigens, as well as acid-susceptible granular, reticular, fungoid, and rod-like forms were also found in considerable quantities.
About the Authors
A. P. Lysenko
Институт экспериментальной ветеринарии им. С. Н. Вышелесского, г. Минск
Belarus
Belarus
V. V. Vlasenko
Винницкий государственный аграрный университет, г. Винница
Belarus
Belarus
A. P. Lemish
Институт экспериментальной ветеринарии им. С. Н. Вышелесского, г. Минск
Belarus
Belarus
T. P. Novik
Минский государственный медицинский университет, г. Минск
Belarus
Belarus
E. A. Mikhalevich
Институт экспериментальной ветеринарии им. С. Н. Вышелесского, г. Минск
Belarus
Belarus
I. G. Vlasenko
Винницкий торгово-экономический институт Киевского национального торгово-экономического университета, г. Винница
Belarus
Belarus
References
1. Авдиенко В. Г., Бабаян С. С., Гусева А. Н. и др.Количественные, спектральные и серодиагностические характеристики антимикобактериальных IgG-, IgM- и IgA-антител у больных туберкулезом легких // Пробл. туб. - 2006. - 10. - С. 47-55.
2. Бондарев И. М., Гулевская С. А., Немсадзе М. Н. и др. Морфология начальных этапов L-трансформации микобактерий туберкулеза при сканирующей электронной микроскопии // Пробл. туб. - 1976. - № 12. - С. 55-61.
3. Лысенко А. П., Архипов И. Н., Лемиш А. П. и др. Особенности антигенного состава измененных форм микобактерий туберкулеза // Пробл. туб. - 2010. - № 10. - С. 54-58.
4. Alavi H., Moscovic E. Immunolocalization of cell-wall-deficient forms of Mycobacterium tuberculosis complex in sarcoidosis and in sinus histiocytosis of lymph nodes draining carcinoma // Histol Histopathol. - 1996. - Vol. 11, № 3. - P. 683-694.
5. Brieger E., Miles J., Cosslet V. Nature and infectivity of granular forms of the bovine tubercle bacillus // Nature. - 1951. - Vol. 168. - Р. 96-100.
6. Daviesa A., Dhillonb A., Youngd M. et al. Resuscitation-promoting factors are expressed in Mycobacterium tuberculosis-infected human tissue // Tuberculosis. - 2008. - Vol. 88, № 5. - Р. 462-468.
7. Geisel O., Netter F., Hermanns W. Specificity of the immunohistochemical demonstration of Mycobacterial antigens // J. Vet. Med. Series B. - 1994. - Vol. 41. - Р. 548-553.
8. Goel M., Budhwar P. Immunohistochemical localization of Mycobacterium tuberculosis complex antigen with antibody to 38 kDa antigen versus Ziehl Neelsen staining in tissue granulomas of extrapulmonnary tuberculosis // Indian. J. Tuberc. - 2007. - Vol. 54. - P. 24-29.
9. Marcova N., Michailova L., Kussovski V., Jourdanova M. Formation of persisting cell wall deficient forms of M. bovis BCG during interaction with peritoneal macrophages in guinea pigs // Electronic J. Biol. - 2008. - Vol. 4, № 1. - P. 1-10.
10. Mukherjee A., Kalra N., Beena K. Immuno-histochemical detection of mycobacterial antigen in tuberculous lymphadenitis // Indian. J. Tuberc. - 2002. - Vol. 49. - P. 213-216.
11. Orrell J., Brett.J, Ivanyi J. et al. Measurement of the tissue distribution of immunoperoxidase staining with polyclonal anti-BCG serum in lung granulomata of mice infected with Mycobacterium tuberculosis // J. Pathol. - 1991. - Vol. 164, № 1. - P. 41-45.
12. Padmavathy L., Rao L., Ramanadhan Sh. Mycobacterial antigen in tissues in diagnosis of cutaneous tuberculosis // Indian. J. Tuberc. - 2005. - Vol. 52. - P. 31-35.
13. Radhakrishnan V., Mathai A., Radhakrishnan N. et al. Immunohistochemical demonstration of mycobacterial antigens in intracranial tuberculoma // Indian. J. ExP. Biol. - 1991. - Vol. 29, № 7. - P. 641-644.
14. Robinson D. Pleomorphic mammalian tumor-derived bacteria self-organize as multicellular mammalian eucaryotic-like organisms: morphogenetic properties in vitro, possible origin, and possible roles in mammalian «tumor ecologies» // Med. Hypothesis. - 2005. - Vol. 64, № 1. - P. 177-185.
15. Slavchev G., Michailova L., Markova N. Stress-induced L-forms of Mycobacterium bovis: challenge to survivability // New Microbiologica. - 2013. - Vol. 36. - P. 157-166.
16. Wiley E., Mulhollan T., Beck B. et al. Polyclonal antibodies raised against BCG, M. duvalii, and M. paratuberculosis used to detect mycobacteria in tissue with the use of immunohistochemical techniques // Am. J. Clin. Pathol. - 1990. - Vol. 94, № 3. - P. 307-312.
17. Zhu Mingli, Zhang Yuanhe, Li Mingjun et al. Comparison of five methods for detection of Mycobacterium tuberculosis and their L-forms in peripheral blood of pulmonary tuberculosis and lung cancer patients // J. Bengbu Med. College. - 2001. - 01.
Review
For citations:
Lysenko A.P., Vlasenko V.V., Lemish A.P., Novik T.P., Mikhalevich E.A., Vlasenko I.G. DETECTION OF TISSUE MYCOBACTERIUM TUBERCULOSIS BY DIFFERENTIATING IMMUNOPEROXIDASE STAINING. Tuberculosis and Lung Diseases. 2014;(10):50-54. (In Russ.) https://doi.org/10.21292/2075-1230-2014-0-10-50-54
Views:
884
Email this article 